ABSTRACT
Objective To compare the hemostasis effect of Sanguisorbae Radix (SR) and charred Sanguisorbae Radix (CSR) before and after baking.Methods Totally 60 Kunming mice were randomly divided into six groups:control group,Yunnan Baiyao (positive drug group,0.667 g/kg),SR high and low dose (8,2 gcrude drug/kg) group,and CSR high and low dose (8,2 gcrude drug/kg) group.Mice were continuously ig with relatively drug once a day for 3 d.The bleeding time and clotting time were tested 1 h after the last administration,the prothrombin time (PT),thrombin time (TT),and activated partial thromboplastin time (APTT) were detected by blood coagulation analyzer,and the number of platelet was count.Results Compared with control group,SR of high dose,and CSR of high and low doses can obviously shorten the bleeding time,clotting time,PT,TT,and APTT.SR of high and low doses and CSR of high dose can elevate the blood platelets count.Compared with SR high dose group,CSR of high dose can obviously shorten the PT,TT,bleeding time,and clotting time,but could not be statistically significant on the blood platelets count and APTT.Conclusion SR and CSR have different hemostasis mechanisms,the function of hemostasis was more effective after charcoal by baking.
ABSTRACT
OBJECTIVE: To compare the hepatotoxicity which induced by rifamycin sodium and isoniazid by alone or combination in mice. METHODS: Forty mice were divided randomly into 4 groups: the sodium chloride group, the rifamycin sodium group, the isoniazid group and the drug - combination group. The drugs were administered to mice by i. p. or i. g. once daily for 8 days. Each mouse was killed by ophthalmectomy. The blood was collected and the liver was excised immediately. The activity of serum ALT and AST was measured and the liver index in mice was calculated. Light microscope was used to observe the histopathological changes of the hepatic cells. RESULTS: The liver index and the activity of serum ALT and AST increased in all groups expect the sodium chloride group( P